Adipoqcre mt/mg idtr mice

Author: ibpn

August undefined, 2024

Web007576 STOCK Gt(ROSA)26Sor tm4(ACTB-tdTomato,-EGFP)Luo /J mT/mG (also called ROSA mT/mG ) is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages … WebSep 7, 2024 · Adiponectin-Cre (JAX#010803), Rosa-iDTR (JAX#007900), and Lkb1 f/f (JAX#014143) were obtained from The Jackson Laboratory. Hdac4 f/f and Ucp1-Cre (JAX#024670) mice were provided by Drs. Eric Olson and Evan Rosen. Mice were in the C57Bl/6J background, and both sexes were analyzed in this study.

Unspecific DNA recombination in AdipoqCre-ERT2 - PubMed

WebSep 26, 2024 · Next, we assessed the effects of deleting Mks to understand their impact in acute thrombocytopenia using Pf4 (CXCL4)-cre; iDTR mice. 6,7 Three days after DT induction, Mks were depleted in Pf4-cre +; iDTR mice, but the number of platelets in the circulation was not reduced in Mk-deficient mice compared with DT-treated Cre − … WebThe Foxl2-CreERT2;mT/mG mouse model allowed us to trace any regeneration of oocytes under physiological conditions that leads to the formation of new follicles through recruitment of nascent follicular somatic cells. smother season 1

Lysozyme M-positive monocytes mediate angiotensin II-induced …

WebAdipoqCre+;mT/mG and AdipoqCreER+;mT/mG mice given tamoxifen 7 days before analysis. (B) Flow cytometry plot of Pdgfra colocalization in cell populations from … WebSep 4, 2024 · While mGFP expression was minimal in adipocytes of untreated Adipoq-CreER; mT/mG mice (0.17% of perilipin+ cells expressed mGFP) (Supplementary Fig. … We would like to show you a description here but the site won’t allow us. WebMay 20, 2005 · Our results validate the use of iDTR mice as a tool for cell ablation in vivo. ... (0.4 mg/ml; Roche) and optical density (OD) measurements at 405 nm versus 570 nm were compared to standard curves ... rizoma action black indicator

Transgenic Strategies to Study Podocyte Loss and Regeneration.

028020 - B6.FVB-Tg(Adipoq-cre)1Evdr/J

WebSep 2, 2024 · Apln-CreERT, Cdh5-CreERT mice, the cell specific membrane (m) fluorescent report mice.a The schematic chart illustrates the generation of CreERT:mT/mG-het mice.b The schematic chart illustrates the mT/mG mouse construct for expression of membrane green eGFP (mG) following tamoxifen administration to remove the red mtdTomato (mT) … WebMay 18, 2015 · Wanner et al. crossed the iDTR strain to hNPHS2.rtTA, TetO.Cre, and mT/mG mice to specifically target podocyte ablation, in a dose-dependent manner, and determine the influence of acute podocyte loss on podocyte regeneration (Figure 3(b)). smother seizoen 2WebSep 19, 2024 · By 9 weeks, mice with podocyte-specific deletion of Vps34 had developed early proteinuria, progressive glomerulosclerosis, and renal failure. ... Autophagy as a Therapeutic Target for Chronic... smother season 2

"Webfrom AdipoqCre+;iDTR mice relative to the same AdipoqCre-;iDTR mice (n = 3-4 mice for each condition and time point). (E-F) Quantification of T cell subsets (E) and the ... from AdipoqCre+;mT/mG mice co-stained with fibrillin or fibronectin. Arrow indicate GFP+ cells that colocalize with ECM proteins. Scale bars, 100μm. (G) Schematic and ... " - Adipoqcre mt/mg idtr mice

Adipoqcre mt/mg idtr mice

028020 - Adipoq-Cre Strain Details - The Jackson …

WebCreERT2xTDTO mice, we observed the previously described Cre leakage without TAM administration. Therefore, we aimed at analysing non-induced DNA recombination in Adipoq-CreERT2xTDTO mice in a more detailed manner using mice of different genotype, sex and age. Importantly, we found that leakage of Cre-ERT2 recombination in Adipoq … WebApr 16, 2024 · To determine if the Adipoq promoter is active in other cell types prior to injury, we analyzed GFP+ expression in AdipoqCre+; mT/mG mice and AdipoqCreER; mT/mG …

Did you know?

WebAim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ERT2), which revealed age, sex and genotype as factors impacting on non-induced Cre recombination. ABSTRACT Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to … WebDec 17, 2015 · Human diphtheria toxin receptors (DTR) were selectively expressed in parathyroid cells by using mice that carry an iDTR allele. 24 These mice were mated to mice that express the Cre allele under the control of the human 5.5-kb PTH promoter, which is active only in parathyroid cells. 23 In the resulting PTHcre-iDTR mice, parathyroid …

WebJan 25, 2024 · Male 2-mo-old Pdgfrα mT/mG or Pdgfrα iDTR;mT/mG mice were sequentially administered TM and DTX (TM: 150 mg·kg-1 per day for 5 d; DTX: 45.7 μg·kg-1 per day for 3 d starting 10 d after TM). WebMay 17, 2024 · Lineage negative (CD45- & CD31-) cells were isolated from uninjured skin and day 5 dorsal skin wound beds of 7-week-old AdipoqCre; mT/mG mice using FACS. This experiment describes multiple subsets of wound bed myofibroblasts and identifies that Adipoq-traced cells contribute to the myofibroblast pool in wound beds. Overall design

WebJun 20, 2014 · Adipocyte dysfunction is believed to underlie the development of metabolic disease in obesity. Hence, understanding how one might beneficially manipulate adipose … WebSep 4, 2024 · While mGFP expression was minimal in adipocytes of untreated Adipoq-CreER; mT/mG mice (0.17% of perilipin+ cells expressed mGFP) (Supplementary Fig. 4a) or in APs from Adipoq-Cre; mT/mG...

WebOct 1, 2010 · Adipoq encodes adiponectin, an adipokine specifically expressed in differentiated adipocytes. Tamoxifen treatment induced almost complete recombination in white adipose tissue of the AdipoqCreER... smothers elementaryWebThe following mice (catalog numbers are from Jackson Laboratory, Bar Harbor, ME, USA) were obtained: ROSAmT/mG mice (007676),(22) PTH-Cre mice (005989),(23) and Cre-inducible DTR transgenic mice (iDTR) mice (007900).(24) Genotyping was performed on genomic DNA isolated from tails, using previously publishedprotocols ... smother season 2 episode 6WebNational Center for Biotechnology Information rizoma mirror block offsWebSep 20, 2011 · Selective ablation of lysozyme M-positive (LysM(+)) myelomonocytic cells by low-dose diphtheria toxin in mice with inducible expression of the diphtheria toxin receptor (LysM(iDTR) mice) reduced the number of monocytes in the circulation and limited ATII-induced infiltration of these cells into the vascular wall, whereas the number of ... rizoma bar ends ducatiWebJun 4, 2024 · To determine whether the Adipoq promoter is active in other cell types before injury, we analyzed GFP+ expression in AdipoqCre+;mT/mG mice and … rizoma pro tapered racing footpegsWebApr 30, 2024 · To date, a dozen mouse models for mast cell deficiency have been reported. However, mice with a specific depletion of all populations of mast cells have not been reported. We generated knock-in mice, termed Mcpt5 / Cma1DTR mice, expressing human diphtheria toxin A (DT) receptor under the endogenous promoter of Mcpt5 (also known … smothers elementary schoolWebHere we describe mT/mG, a double-fluorescent Cre reporter mouse that expresses membrane-targeted tandem dimer Tomato (mT) prior to Cre-mediated excision and … smothers elementary school dc