Bioline tetro cdna synthesis kit

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August undefined, 2024

WebAug 24, 2024 · Complementary DNA (cDNA) synthesis was performed using the Bioline Tetro cDNA synthesis kit and random hexamers (product number: BIO-65042) according to manufacturer protocols. The presence of cDNA in the reaction was confirmed (approx. 200 ng µl −1 per sample) using a NanoDrop 1000 spectrophotometer. 2.3. Primer design WebSnippet: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro …

cDNA Synthesis for qPCR Experiments

WebThe Tetro cDNA Synthesis Kit is optimized for RT reactions using a wide range of total RNA amounts (10 pg -2 μg), such that long and low abundance cDNAs can be detected … WebBioline …cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including… Related Products: Isolate Ii Rna … camera authentication

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WebAdditionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Applications RT-qPCR End-point RT-PCR Northern, dot and slot blotting Array analysis Poly A+ RNA selection RNA-Seq WebThe product portfolio comprises more than 300 reagents and kits, many of them proprietary, for molecular biology and cell analysis. Bioline is an ISO 13485 certified company and one of the world’s few manufacturers of … WebMar 15, 2013 · Final reverse transcription was incubated at 30°C for 10 min, then at 42°C for 60 min followed by inactivation at 95°C for 5 min. Reverse transcription using Bioline’s Tetro cDNA synthesis kit (#BIO-65042, MMLV-based RTase, RNaseH +, Bioline (Aust) Pty Ltd, Alexandria, Australia) was performed as follows: a final volume of 10 µl … camera attachments for phones

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Bioline Cdna at Thomas Scientific

WebAug 5, 2016 · Bioline's Tetro cDNA synthesis kit was used to synthesis first strand cDNA using Salmonella enterica total RNA to quantify gene expression. A total of 1 microgram RNA was used and at the end of the … WebMar 31, 2024 · A Tetro cDNA synthesis kit (catalogue no. BIO-65043, Bioline, London, UK) was used to synthesise cDNA using random primers, while following the manufacturer’s instructions. Two genes from each condition (YPD-28 °C, YPD-16 °C, and YP + glycerol-16 °C) showing significant transcriptional changes in RNAseq dataset were picked for further ... coffee mugs huckberryWebThe double-stranded cDNA was synthesized using a Tetro cDNA Synthesis Kit (Bioline, London, UK). ... Quantitative real-time PCR was performed using the SensiFAST™ SYBR ® No-ROX kit (Bioline, London, UK) in a qTOWER3 G real-time thermal cycler (Analytik Jena, Jena, Germany) with beta actin (β-actin) as the housekeeping gene. cDNA was diluted ... camera-aware proxy

"WebChemical Synthesis; Protein/Peptide Services; Custom mRNA Synthesis Service; Sequencing Analyzed Services; COVID-19. COVID-19 Rapid Tests; COVID-19 Detection Kits; COVID-19 Sample Collection & NA-Isolation; COVID-19 Proteins, Vectors & Plasmids; COVID-19 Antibodies; ... Tetro cDNA Summary Kit. " - Bioline tetro cdna synthesis kit

Bioline tetro cdna synthesis kit

WebThe kits are suitable for DNA templates or RNA templates following reverse transcription with the Bioline Tetro cDNA Synthesis Supermix. The SensiFAST SYBR Hi-ROX and Lo-ROX Kit contain premixed ROX and the SensiFAST SYBR & fluorescein contain premixed fluorescein, for optional use as a passive reference. WebJan 31, 2013 · cDNA synthesis was performed with Tetro Reverse Trancriptase kit (Bioline Ltd.) according to the manufacturer’s recommendation. Quantitative PCR was performed using real-time PCR (ABI PRISM 7900, Applied Biosystems). ... reverse transcription reaction using the designed miRNA specific stem loop-RT primer and …

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WebMar 9, 2016 · I have used Bioline's Tetro cDNA synthesis kit for synthesis of cDNA using random hexamers as primer with E. coli RNA samples to measure gene expression by quantitative real time PCR. The … WebSpecification Name Specification Value; Package Contents: 5X RT Buffer, Reverse Transcriptase (200 U/µl), RNase Inhibitor (10 U/µl), dNTP Mix 10 mM Total, Oligo (dT)18 …

WebJan 6, 2024 · Subsequently, the Tetro cDNA Synthesis Kit was used to convert all RNA samples to cDNA in a 96-well plate using T 100™ Thermal Cycler (Bio-Rad). The qPCR reactions were started by adding 10 ng cDNA to 5 μl SensiFAST™ SYBR and 500 nM forward and reverse primers (Table 3) in a 384-well plate and in duplicates. WebSensiFAST™ cDNA Synthesis Kit Bioline To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST cDNA Synthesis Kit. Efficient – high-target affinity, coupled with a novel TransAmp buffer system for improved yield of full-length cDNA Unbiased – optimized mix of random hexamers… Related Products: Cdna …

WebThe Tetro cDNA Synthesis Kit is optimized for RT reactions using a wide range of total RNA amounts (100pg-2_g), such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis. The Kit contains oligo (dT)18 and random hexamer primers together with control RNA template. See Full Product Listings > About Biosave WebEpiNext cTIP (CUT&Tag In-Place)-Sequencing Kit Description: The EpiNext™ cTIP (CUT&Tag In-Place)-Sequencing Kit is a complete set of reagents required for carrying out a ... Quantity: 12 reactions/ 24 reactions Applications: Immunoprecipitation, Library Construction Supplier Page Sign In or Register to view pricing Compare Product + See …

WebBIOLINE - PRICES VALID FROM 1-01-2024 TO 31-12-2024 Product Name Pack Cat No: LIST PRICE Size INR INR MyTaq DNA Polymerase 500 units BIO-21105 MyTaq DNA Polymerase 2500 units BIO-21106 MyTaq DNA Polymerase 5000 units BIO-21107 MyTaq Mix, 2x 200 Reactions BIO-25041 MyTaq Mix, 2x 1000 Reactions BIO-25042 MyTaq …

http://www.biosave.com/products/tetro-cdna-synthesis-kit-bio-65043 camera at traffic lightsWebSnippet: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro cDNA synthesis kit, both according to the manufacturer's instructions. The cDNA was then quantitated by PCR using Maxima SYBR Green master mix (Thermo) and a … camera avec application smartphoneWebThe first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA. Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-5 μg), such ... camera authentic storeWebTetro cDNA Synthesis Kit (Bioline Reagents) RNaseZap® RNase Decontamination (Ambion®) DEPC-treated water (Ambion®) DNA LoBind 1.5 mL Tubes, PCR clean (Eppendorf) ART® self-sealing barrier pipette tips (Sigma-Aldrich) Eppendorf Mastercycler® PCR Cycler (Eppendorf) StepOnePlus™ Real-Time PCR (Applied Biosystems®) camera azurewave driver windows 1WebISOLATES SECONDARY Genomic DNA Kit your designed for the rapid and efficient isolation of highly pure genomic DNA uses the latest constructions in silica membrane spin column technology. In views our range of antigens … camera babymoov touch screenWebThe Tetro TM cDNA Synthesis kit has a broad dynamic range from 10 pg up to 2 ug of RNA. Several other reagents for RNA analysis are available, such as TRIsure TM . … camera autonome sans fil wifiWebThe Tetro cDNA Synthesis Kit is optimized for RT reactions using a wide range of total RNA amounts (10 pg -2 μg), such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis. The Kit contains oligo (dT)18 and random hexamer primers. The dNTPs included in the kit are manufactured by Bioline and are 99% pure. coffee mugs huntington beach