Fitc偶联annexin-v凋亡检测试剂盒

WebProduct overview. Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs ... Web北京义翘神州（Sino Biological, Inc.）自主研发的高品质 Annexin V/7-AAD 凋亡检测试剂盒以 Annexin V 联合 7-AAD/PI 的双染法（提示 ...

Annexin V-FITC/PI细胞凋亡检测试剂盒-爱必信（absin）官网

WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. Web一般描述. Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞，并使用流式细胞仪进行定量。. AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯（FITC）偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。. 该试剂盒包含碘化丙啶 (PI)，用于标记细胞膜完全破坏 … ontkalker koffiemachine action https://lynxpropertymanagement.net

细胞凋亡检测 凋亡信号 - BD Biosciences

WebThe Annexin V-FITC Kit contains reagents for the simultaneous fluorescent detection of apoptotic, necrotic, and dead cells through the specific binding of FITC-labeled Annexin V to phosphotidylserine (PS). The kit consists of Annexin V-FITC, Annexin V Binding Buffer, and Propidium Iodide Solution. WebStaining. 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. Webannexin v- fitc/pi 凋亡检测试剂盒.由晶抗生物整理，包括annexin v- fitc/pi 凋亡检测试剂盒.使用说明书、应用范围、使用注意事项及操作步骤等资讯。 欢迎莅临上海晶抗生物工程有限公司官方网站！ ontknopen

LinKine™ FITC偶联标记试剂盒，高效又简便！ 每日生物评论

Category:Annexin V/7-AAD凋亡试剂盒 义翘神州 - Sinobiological

Tags:Fitc偶联annexin-v凋亡检测试剂盒

Fitc偶联annexin-v凋亡检测试剂盒

eBioscience™ Annexin V 细胞凋亡检测试剂盒 - Thermo …

WebAnnexin V是检测细胞早期凋亡的常用方法之一。实验操作虽然不算难，想要获得漂亮的流式结果图却并不容易，任何一个细节的疏忽都可能导致实验结果不理想。 本篇文章，带大家了解实验过程中的注意事项，及不能忽视的实验细节。 01 实验原理 Web悬浮细胞收集：离心5分钟；贴壁细胞：用不含EDTA的胰酶消化收集后（注：胰酶消化时间不宜过长，否则会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合），于室温2000rpm离心5～10分钟，收集细胞；. （3）细胞洗涤：用预冷1×PBS（4℃）重悬细胞一次，2000rpm离心5 ...

Fitc偶联annexin-v凋亡检测试剂盒

Did you know?

Web英文名字 LinKine™ FITC Labeling Kit. 中文名称 LinKine™ FITC 偶联试剂盒. 产品货号 KTL0210. 规格 3*20ug. 背景资料： 异硫氰酸荧光素（FITC）是荧光素的衍生物，广泛应用于流式细胞术。 FITC是用异硫氰酸酯反应性基团（-N = C = S）官能化的原始荧光素分子，取代结构底部 ... Web一般描述. Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞，并使用流式细胞仪进行定量。. AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯（FITC）偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。. 该试剂盒包含碘化丙啶 (PI)，用于标记细胞膜完全破坏 …

WebAnnexin V-FITC Microscopy Staining Protocol. 1. Wash cells twice with 1X PBS. 2. Wash cells once with 1X Annexin V Binding Buffer. 3. Stain cells with Annexin V-FITC diluted 1:10 in 1X Annexin V Binding Buffer for 15 min at RT. We typically use 2 ml total volume per Labtek II chamber slide (i.e., 200 µl Annexin V-FITC and 1.8 ml 1X Binding Buffer. WebMay 27, 2024 · Annexin V-FITC细胞凋亡检测试剂盒(Annexin V-FITC Apoptosis Detection Kit)是用FITC标记的重组人Annexin V来检测细胞凋亡时出现在细胞膜表面的磷脂酰丝氨酸的一种细胞凋亡检测试剂盒。可以使用流式细胞仪、荧光显微镜或其它荧光检测设备进行检测。

WebAnnexin-V（膜联蛋白-V）是一种分子量为35-36KD的Ca2+ 依赖性磷脂结合蛋白，能与PS高亲和力结合。用荧光素（FITC、Alexa Fluor488等）标记的 Annexin-V 作为探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。另外，碘化丙啶(propidine iodide, PI)是一种核酸染料，它不 ... WebBioLegend

WebFlow Cytometric Analysis of Annexin V-FITC staining. Jurkat cells were untreated (left panels), treated for 5 hours (middle panels) or 18 hours (right panels) with 4μMCamptothecin (Sigma,Cat.No C9911). Cells were incubated with Annexin V-FITC and 7-AAD and analyzed by flow cytometry. Untreated cells were primarily Annexin V-FITC …

WebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to … ontkalker philips koffiemachineWeb3. 加入5-15µg纯化的重组Annexin V。注意，不同的细胞系，以及凋亡的不同阶段，其Annexin V位点 饱和所需要的纯化的重组Annexin V含量不同。某些情况下，为达到最好效果，可以减少细胞数量， 0.5´105个细胞加5-15µg纯化的重组Annexin V。 4. 轻轻混匀，室温反应15分钟。 5. ont kcomWebDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry: Kit Contents: Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X … ontketen je brein theo compernollehttp://www.bio-review.com/fitc-labeling-kit/ ios show reminders on lock screenWebFITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. ontknoping f1WebMethod. 1. Cell incubation with Annexin V-FITC. 1.1 Induce apoptosis via the desired method. 1.2 Collect 1–5 x 10 5 cells by centrifugation. 1.3 Resuspend cells in 500 µL of 1X Annexin V binding buffer. 1.4 Add 5 µL of annexin V-FITC and 5 µL of propidium iodide (PI, optional). 1.5 Incubate at room temperature for 5 min in the dark. ios show phone numberWeb概述. 本试剂盒提供快速而简单的偶联过程，FITC是目前常用的荧光染料之一，通过抗体或蛋白的伯胺（即赖氨酸残基）与FITC的硫碳胺基结合，形成FITC-蛋白质结合物，即FITC标记抗体或蛋白。. 标记抗体或蛋白可应用于ELISA，Western blot，IF和 FACS分析等。. ios show touches