Phospho 4ebp1 antibody

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August undefined, 2024

WebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide. WebProduct Description. Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. The association between 4EPB1 and eukaryotic ...

Recombinant Anti-eIF4EBP1 (phospho S65) antibody [EPR23889 …

WebProduct name Anti-eIF4EBP1 (phospho T70) antibody [EPR654 (2)A] See all eIF4EBP1 primary antibodies Description Rabbit monoclonal [EPR654 (2)A] to eIF4EBP1 (phospho T70) Host species Rabbit Tested applications … WebListed below are anti-4EBP1 antibodies from multiple suppliers. 4EBP1 is a reported alias name for the human gene EIF4EBP1, or 'eukaryotic translation initiation factor 4E binding protein 1'. ... Immunotag™ Phospho-4EBP1(Ser65/Thr70) Antibody. Applications: WB, IHC; Reactivity: Hu, Ms, Rt; Conjugate/Tag: Unconjugated, Biotin, FITC, HRP, Alexa ... biography dennis waterman

Anti 4EBP1 (C-Terminal) Antibody Bio-Rad

WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence. WebPhospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (63) 4EBP1 antibodies Add To Cart Save to list Performance Guarantee Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (1) WebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ... biographydesigner marc fisher

Phospho-4E-BP1 (Thr70) Antibody from Cell Signaling Technology

Anti-eIF4EBP1 (phospho T36) antibody (ab47365) Abcam

WebJun 25, 2010 · Anti-phospho Drosophila S6K, anti-S6K, anti-phospho S6K, anti-Akt, anti-phospho Akt, and anti-phospho 4EBP1 antibodies were from Cell Signaling. Anti-Myc, anti-HA, and anti-FLAG antibodies were from Santa Cruz Biotechnology, Covance, and Sigma, respectively. ... stimulation for 30 min. Phosphorylation and protein levels of dS6K were … WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E … biography desmond tutuWebAbcam - antibodies and reagents supplier, find any antibody Hello. We're improving abcam.com and we'd welcome your feedback. ... 4ebp1 35 results for 4ebp1 ... Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] - BSA and Azide free (ab283288) daily care of horses

"Web4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD. Distribution Cytosol and nucleus/ubiquitously expressed Function Regulation of global translation Biology Area Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction Molecular Family Phospho-Proteins Antigen References " - Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb 2855S from

WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of mouse 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E.

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WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ...

WebAnti p-eIF4EBP1 (Ser65/Thr70) antibody, Anti BP1 antibody, Anti BP 1 antibody, Anti eIF4EBP1 (phospho Ser65/Thr70) antibody, Anti PHAS I antibody, Anti 4EBP1 antibody, Anti PHASI antibody, Anti 4E-BP1 antibody, Anti eIF4E-binding protein 1 antibody, Anti eIF4EBP1 (phospho S65/Th70) antibody, Anti 4E BP1 antibody, Anti eIF4E binding protein 1 … Webp-4E-BP1 Antibody (62.Ser 65): sc-293124. Western blot analysis of 4E-BP1 phosphorylation in HeLa (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates. Description Gene Info Datasheets and Protocols The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA.

WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … WebSep 7, 2024 · 4E-BP1 Antibody (P-1) is recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC (P) and ELISA. Anti-4E-BP1 Antibody (P-1) is available conjugated to agarose for IP; HRP for WB, IHC (P) and ELISA; and to either phycoerythrin or FITC for IF, IHC (P) and FCM.

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WebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. biography dennis weaverWebView Rabbit Polyclonal anti-4EBP1 [p Thr46, p Thr37] Antibody (NBP3-13169). Validated Applications: WB. Validated Species: Human, Mouse, Rat + more. Validated by: Biological Strategies, Genetic Strategies. daily care plan for dementia patientsWebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the … biography dewey decimalWeb人促胃液素受体(GsaR)ELISA试剂盒 Mouse proliferating cell nuclear antigen antibody,PCNA ELISA试剂盒规格： 96T/48T. ... eIF4EBP1/4EBP1 eIF4E结合蛋白抗体 0.1ml 沙氏葡萄糖琼脂Sabouraud Dextrose Agar ... 磷酸化4E结合蛋白1抗体 0.1ml 沙氏葡萄糖肉汤Sabouraud Dextrose Broth phospho-eIF4EBP1(Thr37/46) 磷酸 ... daily care portalWebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb daily care of picc lineWebThe Simple Western TM antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. biography dewey decimal systemWebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … daily care of newborn baby