Potentiometric dye tetramethylrhodamine
WebTetramethylrhodamine methyl ester (TMRM) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.1,2,3 It has two excitation peaks at 515 and 555 nm and an emission peak in the red-orange range (575 nm). Due to the polarization ... Web23 Dec 2016 · ( B) Determination of mitochondrial membrane potential with TMRM staining. HL60 cells were treated for 20 hours with the following compounds: 5 mM metformin, 100 μM phenformin (Phen), 8 nM piericidin A (Pier), 5 nM antimycin A (AntA), 1 nM oligomycin (Oligo), 10 μM FCCP (positive control), and 5 μM syrosingopine.
Potentiometric dye tetramethylrhodamine
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WebTMRM Potentiometric Probes TMRM Structure and Spectra of TMRM ⋅ Packaging ⋅ Order information ⋅ Citations Structure and Spectra of TMRM Absorbance, emission peaks in … WebTetramethylrhodamine ethyl ester (TMRE) and 2- (4- (dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) are rapidly and reversibly taken up by live cells (Loew …
Web2 Mar 2024 · The potentiometric characteristics of the proposed potentiometric sensor were examined at the detection of citrates in the real-world samples, compared with results for PNR simple electrode, and corresponded with the reference capillary electrophoresis and literature-based spectrophotometric method. ... In situ polarization modulation—Fourier ... WebThe potentiometric dye, Tetramethylrhodamine methyl ester (TMRM) has been extensively used with fluorometry or optical microscopy to evaluate the electric potential across …
Web16 Nov 2024 · Furthermore, total probe accumulation and fluorescence intensity of ΔΨm-indicating probes, including tetramethylrhodamine methyl ester (TMRM), is also a … Webthe potentiometric dye Tetramethylrhodamine (TMRM), revealed mitochondrial depolarization following ATP synthase inhibition by oligomycin in OPA1 M/M cells, …
WebThermo Fisher potentiometric dye tetramethylrhodamine methyl ester tmrm Potentiometric Dye Tetramethylrhodamine Methyl Ester Tmrm, supplied by Thermo Fisher, used in …
WebTetramethylrhodamine B isothiocyanate isomer R (40 mg; Sigma) and 100 mg of either amino-dextran (to make the fixable tracer RDA) or carboxy-dextran (to make the … hung ceiling detailWebInterestingly, Δψmis higher in many cancer cells comparted to healthy noncancerous cell types, providing a unique metabolic marker. This feature has also been exploited for … hung dance pianoWeb( D–G) PASMCs were transfected with photoactivatable, mitochondrial-targeted green fluorescent protein (Mito-PA-GFP) and then loaded with a potentiometric dye, tetramethylrhodamine (10 nM at 37°C for 30 min). Mitochondrial networking was evaluated with photoactivating Mito-PA-GFP as described in the M ethods section in the online … capitalism on pollutionWeb1 Apr 2016 · DAPI can be combined with Annexin V(FITC)and the potentiometric fluorescent dye, tetramethylrhodamine methyl ester (TMRM), which measures mitochondrial … hung daggerWebMetrics Abstract Digital imaging (confocal microscopy) and a slow potentiometric dye (tetramethylrhodamine methyl ester) were used to assess the resting membrane potential ( V m) of murine neuroblastoma cells (N1E-115). … hung blu rayWeblipophilic, cationic fluorescent redistribution dyes such as tetramethylrhodamine ethyl (TMRE) and methyl (TMRM) esters and 5,5’,6,6’-tetrachloro-1,1’,3,3’- ... electrochemical … capital oneusaaWebPotentiometric dyes. The anionic oxonol dye, bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBa-C4[3]; Molecular Probes, Eugene, OR), was chosen for flow cytometry … hung fat trading asien livs ab